At least 163 Palestinians and six Israeli police officers have been hurt in clashes in Jerusalem, Palestinian medics and Israeli police say.
Most were injured at the Al-Aqsa Mosque, where Israeli police fired rubber bullets and stun grenades as Palestinians threw stones and bottles.
Tensions have been rising over the potential eviction of Palestinians from land claimed by Jewish settlers.
The Red Crescent has opened a field hospital to treat the wounded.
The Al-Aqsa mosque complex in Jerusalem's Old City is one of Islam's most revered locations, but its location is also the holiest site in Judaism, known as the Temple Mount.
The site is a frequent flashpoint for violence, which unfolded again on Friday night after thousands had gathered there to observe the last Friday of the Muslim holy month of Ramadan.
Israeli Police said they had used force to "restore order" due to the "rioting of thousands of worshippers" after evening prayers.